Fig. 4: Inhibition of βFaar expression results in β-cell apoptosis.
From: The long non-coding RNA βFaar regulates islet β-cell function and survival during obesity in mice
βFaar overexpression plasmid (oe-βFaar) and βFaar smart silence (si-βFaar) were transfected into MIN6 cells for 48 h. Then the FITC+/PI+ cells were measured by Flow cytometry (a). b, c The both INSULIN and TUNEL positive cells were detected by immunofluorescence in the mice islets (b) and in the human islets (c). Magnification: ×40 or ×20, scale bar, 10 μm or 20 μm. d The protein levels of Pro-Caspase 3, Cleaved Caspase 3, BCL-2, and BAX in the MIN6 cells after transfection for 48 h. e, f The protein levels of Pro-Caspase 3, Cleaved Caspase 3, BCL-2, and BAX in the islet of βFaar-KO mice (e, n = 5) and len-βFaar mice (f, n = 5). g The TUNEL positive β cells in the islets of βFaar-KO mice and len-βFaar mice. Magnification: ×20, scale bar, 20 μm. All experiments above were performed in triplicates, and each group contained three batches of individual samples. The p-values by one-way ANOVA (a–c, g). Data represent the mean ± SD. Source data are provided as a Source data file.
