Fig. 5: hnRNPLL determines ECM1a mRNA splicing and ECM1a-associated signaling and tumor growth. | Nature Communications

Fig. 5: hnRNPLL determines ECM1a mRNA splicing and ECM1a-associated signaling and tumor growth.

From: Extracellular matrix protein-1 secretory isoform promotes ovarian cancer through increasing alternative mRNA splicing and stemness

Fig. 5

hnRNPLL protein expression in ECM1-silenced and ECM1a-overexpressing cells (a), in ECM1a-WT/ECM1a-MT cells (b), and in ECM1a- and ECM1b-cross-transfected cells (c). Overexpression of hnRNPLL in A8i cells (d) promotes ECM1a mRNA splicing, as tested by semiquantitative RT-PCR (e) and quantification (f, data are presented as mean ± SD, n = 3 biologically independent repeats, two-tailed t-test). Silencing of hnRNPLL in A8 cells with specific shRNAs (g) reduces ECM1a mRNA production but upregulates ECM1b mRNA production, as detected by semiquantitative RT-PCR (h) and quantification (i, data are presented as mean ± SD, n = 3 biologically independent repeats, two-tailed t-test). hnRNPLL silencing (j) or overexpression inhibits or enhances 3D cell growth as shown by spheroid quantification (k, data are presented as mean ± SD, n = 3 biologically independent repeats, two-tailed t-test) and formation (l, bars = 400 μm), xenograft tumor growth (m, data are presented as mean ± SD, n = 9v9 mice, two-tailed t-test) and tissues (n) in nine mice, and activation of ECM1-associated signaling molecules (o), respectively.

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