Fig. 7: ABCG1 confers cancer cell stemness and cisplatin resistance through upregulation of CD326. | Nature Communications

Fig. 7: ABCG1 confers cancer cell stemness and cisplatin resistance through upregulation of CD326.

From: Extracellular matrix protein-1 secretory isoform promotes ovarian cancer through increasing alternative mRNA splicing and stemness

Fig. 7

Inhibition rates of various cell lines treated with cisplatin in terms of silencing or overexpression of ECM1, ABCG1, and hnRNPLL (a, data are presented as mean ± SD, n = 3 biologically independent repeats, two-tailed t-test was calculated between A8i-ABCG1 and A8i <upper> or A8i-A <lower>) and IC50 values (b, data are presented as mean ± SD, n = 3 biologically independent repeats, two-tailed t-test) of cisplatin in these cells as detected by CCK-8 assay. c Expression of stem cell transcription factors detected by WB analysis in ECM1-, ABCG1-, and hnRNPLL-silenced or -overexpressing cells. d Numbers of CD117+ and CD326+ cells (left panel, data are presented as ± SD, n = 3 biologically independent repeats, two-tailed t-test) and cisplatin inhibition rates (right panel, data are presented as ± SD, n = 3 biologically independent repeats) after three selection-and-culture cycles from CD117 + cells. e Numbers of CD117 + and CD326 + cells (left panel, data are presented as mean ± SD, n = 3 biologically independent repeats, two-tailed t-test) and cisplatin inhibition rates (right panel, data are presented as mean ± SD, n = 3 biologically independent repeats, two-tailed t-test was calculated between CD117+ and CD326+ <upper> or CD117+/CD326+ and CD117−/CD326−) after four selection-and-culture cycles from CD326+ cells. f A low concentration of cisplatin enriches CD326+ cells to increase cancer cell stemness. Data are presented as mean ± SD, n = 3 biologically independent repeats, two-tailed t-test. g Cisplatin treatment upregulates the expression of stem cell transcription factors in CD326+ cells.

Back to article page