Fig. 2: Robust association of nanoparticles to the BBB. | Nature Communications

Fig. 2: Robust association of nanoparticles to the BBB.

From: Post-capillary venules are the key locus for transcytosis-mediated brain delivery of therapeutic nanoparticles

Fig. 2

a–e Two-photon in vivo images of RI7-L-A550 nanoparticles (red) 2 h after injection into the circulation. Co-injected FITC-dx (green) delineates vessel lumen. Nanoparticles readily associate to vessel walls at pial venules, ascending venules, post-capillary venules, and capillaries. f In contrast to venules and capillaries, nanoparticles do not associate to arterial branches of the brain microvasculature. g Co-injection of both RI7-L-A550 and RI7-L-A488 reveals discrete punctae of both variants of nanoparticles at the vessel walls. h Time-lapse images of laser-extravasated nanoparticles in brain parenchyma. The presence of merged fluorescence signal is because of the fluorescence signal overlap when nanoparticles are in proximity to each other (arrowhead) and not due to nanoparticle fusion or exchange of fluorophores. All panels: pV pial venule, ascV ascending venule, pcV post-capillary venule, cap capillaries, penA penetrating arteriole, pA pial arteriole. ‘zs’ inset indicates Z-stack maximum intensity projection.

Back to article page