Fig. 2: Early TNF production is impaired in MCPIP3-deficient macrophages.
From: The RNase MCPIP3 promotes skin inflammation by orchestrating myeloid cytokine response
a Tnf expression in R848-treated Zc3h12c+/+(blue)/Zc3h12c−/− (red) bone marrow-derived macrophages (BMDMs) was measured by qPCR at indicated timepoints (n = 3 mice per group). b, c TNF(b) and IL-6(c) secretion by R848-treated Zc3h12c+/+/Zc3h12c−/− BMDMs was measured by ELISA at indicated timepoints (n = 3 mice for Zc3h12c+/+; n = 4 mice for Zc3h12c−/−). d–f TNF production by Zc3h12c+/+/Zc3h12c−/− BMDMs (d, n = 7 mice for Zc3h12c+/+; n = 8 mice for Zc3h12c−/−), Zc3h12cfl/fl/Zc3h12cMΦΔ BMDMs (e, n = 3 mice for Zc3h12cfl/fl; n = 5 mice for Zc3h12cMΦΔ), and purified Zc3h12c+/+/Zc3h12c−/− or Zc3h12cfl/fl/Zc3h12cMΦΔ peritoneal macrophages (Per.MΦ) (f, n = 3 mice for Zc3h12cfl/fl; n = 5 mice for Zc3h12cMΦΔ) was measured by intracellular staining after 4 h of stimulation with indicated ligands. g Il12p40 expression in R848-treated Zc3h12c+/+/Zc3h12c−/− BMDMs was measured by qPCR at various timepoints (n = 3 mice per group). h, i IL-12p40 production by Zc3h12c+/+/Zc3h12c−/− BMDMs was measured by intracellular staining after 4 h (h, n = 4 mice per group) or 16 h (I, n = 4 mice for Zc3h12c+/+; n = 3 mice for Zc3h12c−/−) stimulation with indicated ligands. Data are representative of eight (d, e), four (a, b), three (h, i), two (c, f, g) independent experiments (mean ± S.D.; two-tailed Student’s t test). Source data are provided as a Source Data file.
