Fig. 1: Tet enzyme kinetics.
From: Redirected nuclear glutamate dehydrogenase supplies Tet3 with α-ketoglutarate in neurons
a Illustration of αKG and O2-dependent mdC-oxidation to hmdC and further on to fdC and cadC by Tet enzymes, with hmdC being the main oxidation product. FdC and cadC can be finally replaced by unmodified dC. b Western blots against Tet1, Tet2, and Tet3 in nuclear lysate of mESCs compared to murine brain. c MALDI-TOF MS spectra (n = 3 independent experiments, spectra show mean) of a single-stranded DNA oligonucleotide containing mdC (blue and Na+ peak in gray) that is oxidized to hmdC, fdC, and cadC (magenta) by GFP-Tet1cd or GFP-Tet3cd in the presence of increasing αKG concentrations. A.U. arbitrary units, m/z mass to charge ratio. Source data are provided as a Source Data file.
