Fig. 2: Interaction of Tet3 with Gdh in neurons.
From: Redirected nuclear glutamate dehydrogenase supplies Tet3 with α-ketoglutarate in neurons
a Experimental set-up for Tet3-enriched coIP. b Volcano plot after Tet3-enriched coIP in nuclear brain lysate of adult mice (n = 4 biologically independent sample preparations). Interaction partners were analyzed using label-free quantification after LC–MS/MS. Enriched interaction partners (FDR < 0.05, log2 FC > 1) are highlighted in black. c Conversion of glutamate to αKG by Gdh and glutamate to αKG ratio determined by NMR-based metabolomics in murine hippocampus (n = 6 biologically independent animals, bar shows mean, error bars SD). d Western blots detecting Tet3, Ogt, and Gdh after endogenous Tet3-coIP in nuclear brain lysate. e PLA signal (white/orange dots) of Tet3 and Gdh in the dentate gyrus (DG) of the murine hippocampus. Nuclei (blue) were stained with Hoechst and NeuN staining indicates neuronal nuclei. Scale bar is 5 µm. Source data are provided as a Source Data file.
