Fig. 4: Frequency and differentiation status of SARS-CoV-2-specific MHC-I multimer+ T cells. | Nature Communications

Fig. 4: Frequency and differentiation status of SARS-CoV-2-specific MHC-I multimer+ T cells.

From: SARS-CoV-2-specific T cell memory is sustained in COVID-19 convalescent patients for 10 months with successful development of stem cell-like memory T cells

Fig. 4

PBMC samples (n = 15) from individuals with SARS-CoV-2 infection (n = 11) were analyzed by flow cytometry. a Representative flow cytometry plots showing the ex vivo detection of SARS-CoV-2 S269 multimer+CD8+ T cells in the gate of CD3+ T cells. b Scatter plot showing the relationship between DPSO and the frequency of SARS-CoV-2 S269 multimer+ cells among total CD8+ T cells. Samples from the same patient are connected by solid lines. The expression of CCR7, CD45RA, and CD95 was analyzed in SARS-CoV-2 S269 multimer+CD8+ T cells. IAV MP58 multimer+ (n = 5) and CMV pp65495 multimer+ (n = 6) cells from the PBMCs of healthy donors were also analyzed. Representative flow cytometry plots (c) show the proportion of the indicated subsets among multimer+ cells, and scatter plots (d) show the relationship between DPSO and the proportion of the indicated subsets among SARS-CoV-2 S269 multimer+ cells. Samples from the same patient are connected by solid lines. Summary data showing the proportion of the indicated subsets among IAV multimer+ and CMV multimer+ cells are also presented (d). Horizontal lines represent median. e A representative flow cytometry plot (upper) and summary data (lower) showing the percentage of PD-1−TIGIT− cells among SARS-CoV-2 S269 multimer+CD8+ TSCM cells and total SARS-CoV-2 S269 multimer+CD8+ T cells. Statistical analysis was performed using the two-sided Wilcoxon signed-rank test (e).

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