Fig. 2: La(III)-induced endocytosis or La(III) internalized by endocytosis in leaves induced systemic endocytosis.
From: Lanthanum(III) triggers AtrbohD- and jasmonic acid-dependent systemic endocytosis in plants
a The representative CLSM images of Col-0, and the grafted Col-0, chc1-3, and DEX-inducible Venus-CANTH (shoots from Col-0, chc1-3, or DEX-inducible Venus-CANTH were grafted onto Col-0 roots) leaf cells treated with 0, 30, or 80 µM La(III) for 12 h and stained with FM4-64. Bar = 5 μm. b Quantitative analysis of leaf cells with endocytosis in Col-0, the grafted Col-0, chc1-3, and DEX-inducible Venus-CANTH (shoots from Col-0, chc1-3 or DEX-inducible Venus-CANTH were grafted onto Col-0 roots) (data come from a). c The representative CLSM images of Col-0, and the grafted Col-0, chc1-3, and DEX-inducible Venus-CANTH (shoots from Col-0, chc1-3, or DEX-inducible Venus-CANTH were grafted onto Col-0 roots). The four kinds of Arabidopsis leaves were treated with 0, 30, or 80 µM La(III) for 12 h and then roots were stained with FM4-64. Bar = 10 μm. d Quantitative analysis of the average fluorescent area of FM4-64 in Col-0, and the grafted Col-0, chc1-3, and DEX-inducible Venus-CANTH (shoots from Col-0, chc1-3, or DEX-inducible Venus-CANTH were grafted onto Col-0 roots) root cells with endocytosis (data come from c). In (a, c), representative images from six independent measurements and three biological replicates (each replicate represents an independently treated plant) per measurement are represented. In (b, d), values shown are means ± SEM, one-way ANOVA analysis with LSD multiple comparisons test (n = 6, **p < 0.01, n.s.: no significance).
