Fig. 3: The mutant CD63-YA displays different trafficking from CD63-WT and similar trafficking as CD9. | Nature Communications

Fig. 3: The mutant CD63-YA displays different trafficking from CD63-WT and similar trafficking as CD9.

From: Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9

Fig. 3

a Scheme of the structure and C-terminal sequences of CD63-WT and the mutant CD63-YA. b Immunofluorescence of HeLa cells transfected with the RUSH CD63-eGFP or CD63-YA-eGFP plasmids at steady state. Scale bar 10 μm. This experiment was performed once. c Micrographs of HeLa cells co-transfected with CD63-YA-eGFP and CD63-WT-mCherry or CD9-eGFP and CD63-YA-mCherry, and median ± range of the Pearson’s co-localization coefficient over time between eGFP and mCherry. Biotin was added at T = 0. Z-projection of 11 planes. Scale bar 5 μm. CD63/CD63-YA: 3 independent experiments n = 51 cells, CD9/CD63-YA: 2 independent experiments n = 33 cells. 5 fields per experiment where imaged, for a total of at least 10 individual cells to analyze per experiment.

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