Fig. 5: Micro Capture-C (MCC) shows that the ζ-globin gene does not contact the α-globin enhancers in definitive eryrthroblasts.
From: Reactivation of a developmentally silenced embryonic globin gene
MCC from the α-globin promoters (black arrowheads) shows specific contact of these genes with the enhancers, interactions are represented by peaks at the enhancer elements in this track. Conversely, MCC from the viewpoint of the Hba-x promoter (black arrowhead) shows Hba-x does not contact the α-globin enhancers, or indeed any other sites within the α-globin TAD, suggesting it is isolated. MCC from the CTCF-binding site at HS-38 (black arrowhead) confirms this as it shows interaction with the α-globin genes but no contact with Hba-x. ATAC and CTCF binding profiles are shown for orientation. The orientation of CTCF-binding sites is shown by red and blue arrows and the extent of the α-globin TAD is indicated by a double-headed arrow.
