Fig. 6: Lrf and Bcl11a play a role in silencing ζ-globin expression in mice. | Nature Communications

Fig. 6: Lrf and Bcl11a play a role in silencing ζ-globin expression in mice.

From: Reactivation of a developmentally silenced embryonic globin gene

Fig. 6

a ATAC-seq at the α-globin locus in LrfFlox/LrfFlox (LrfFlox), LrfFlox/- (+/−), Lrf−/− (−/−) erythroid cells, Bcl11aΔ10kb/WT(Δ10kb/WT) and Bcl11aΔ10kb/Δ10kb (Δ10kb/Δ10kb) erythroid cells were taken at E12.5–E13.5. The ζ-globin gene is highlighted in blue, tracks from definitive and primitive erythroid cells (E12.5 and E10.5 respectively) are also shown. b, c Single-cell expression of α- and ζ-globin when Lrf and Bcl11a expression have been perturbed. Data are generated from littermates. Each dot represents a single cell, and the median expression is indicated by a horizontal line.

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