Fig. 2: Fluctuation of HIV-1 transcription over short time periods, with and without Tat.
From: Stochastic pausing at latent HIV-1 promoters generates transcriptional bursting
A–F Fluctuations of HIV-1 transcription over 15–20 min periods, with one image stack recorded every 3 s. A, C, E Each graph is a single transcription site; the x-axis represents the time (in minutes) and y-axis represents the intensity of transcription sites, expressed in equivalent numbers of full-length pre-mRNA molecules. B, D, F Each line is a cell and the transcription site intensity is color-coded (scale on the right). A, B High-Tat cells. C, D Low-Tat cells. E, F No-Tat cells. Source data are provided as a Source Data file. G Schematic of a polymerase convoy. Top: a polymerase convoy, with polymerases in orange and the gene represented as a black horizontal arrow. Npol: number of polymerases; tspace: spacing between successive RNA polymerases (in seconds); vel: elongation rate. Bottom: schematics describing the different phases of a transcription cycle (left) and the position of the polymerase convoy on the MS2 tagged gene (right; the green box is theMS2 tag). H Box-plots representing the parameters values of the best-fit models, measured for a set of isolated transcription cycles in each cell line (n = 89, 36, and 59 for High Tat, No Tat, and Low Tat, respectively). tproc is the 3′-end RNA processing time; Npol is the number of polymerases in the convoy; Vel is the elongation rate (in kb/min); tspace is the spacing between successive polymerase (in seconds). The bottom line displays the first quartile, the box corresponds to the second and third quartile, the top line to the last quartile, and the double circle is the median. Small circles are outliers (1.5 times the inter-quartile range above or below the upper and lower quartile, respectively). Source data are provided as a Source Data file.
