Fig. 1: A group of mutants showing reversed gravitropic responses in Physcomitrella patens.

a Schematic diagram of the screening protocol for identifying gravitropic mutants in P. patens. Protoplasts of P. patens were isolated and treated with UV. Next, protonemata were regenerated in light and then grown on vertically oriented culture plates in the dark to record phenotypes. b Gravitropic phenotype of P. patens protonemata. Wild-type Gransden (Gd) strains of P. patens and UV-derived mutants were grown for around 3 weeks vertically in the dark. Scale bars, 3 mm. c Quantification of bending angles of protonemata growing in the dark for 3 weeks and then gravistimulated for 2 weeks. The schematic diagram shows how the angles were measured, and the bending angles are shown in circular histograms. Source data are provided as a Source Data file. In (a–c), arrows labeled with “g” indicate the directions of gravity vectors.