Fig. 4: The localization and movement of GTRC proteins.

a Localization of GTRC on the microtubules (MT) in the tip cells of P. patens protonemata. RFP-TUB was co-expressed with GFP-GTRC in the protonemata, and fluorescence signals were collected by confocal microscopy. The scatterplots show the Pearson’s correlation coefficient (r) between these two fluorescent signals. Scale bar, 5 μm. b Localization of GTRC was dependent on tubulin but not actin. Images were taken after 10 min of oryzalin or latrunculin B treatments, and oryzalin but not latrunculin B treatment disrupted the regular signal patterns of GTRC. Scale bar, 5 μm. More than 10 cells were studied for each treatment, and the results were similar. Oryzalin and latrunculin B disrupt the microtubules and actin filaments, respectively, as shown in Supplementary Fig. 7. c GTRC moves along MT in the minus-end direction. Left, processive movement of GTRC recorded by time-lapse photography using spinning disk confocal microscopy at 250 ms intervals. Red arrows mark the GFP-GTRC proteins moving along MT. Right, kymographs for the movement of GFP-GTRC. d Velocity of moving GFP-GTRC signals. The mean value is shown with standard deviation and examined sample size. Source data are provided as a Source Data file.