Fig. 5: Functional domain analysis of GTRC protein.

a Schematic diagrams of full length and truncated constructs of GTRC. GFP was fused to the N-terminus of each fragment. b Complementation and fluorescence analyses of truncated GTRC. Upper, various constructs of GTRC were used to replace the genomic GTRC in gtrC-16 via homologous recombination, and then the protonemata were grown on vertical plates. Scale bars, 3 mm. Lower, subcellular localization of GFP-fused full length or truncated GTRC proteins. Scale bars, 5 μm. White dashed lines depict the contours of the tip cells. For each genotype, the fluorescence of more than 10 tip cells was collected, and the results were similar. c Schematic diagrams of full length and chimeric constructs of GTRC and KCHa. 3×FLAG (F) was fused to the N-terminus of each fragment. d Functional analyses of chimeric proteins between GTRC and KCHa. Various constructs were used to replace the genomic GTRC in gtrC-16 via homologous recombination, and then the protonemata were grown on vertical plates. Scale bars, 2 mm. The details of the constructs and genotypes for the P. patens lines are shown in Supplementary Fig. 6.