Fig. 6: Transgene silencing is impaired in morc mutants.
From: Arabidopsis MORC proteins function in the efficient establishment of RNA directed DNA methylation
a Flowering time of T1 plants in wild-type, nrpe1-11, and morc hex backgrounds as indicated by the leaf number. The green dots represent the four late flowering plants propagated to the T2 generation. b qRT-PCR of FWA expression of four independent T1 plants in hex backgournd (crimson) relative to that in nrpe1 background (gray). Error bars represent the mean ± s.d. of 3 technical replicates. c McrBC analysis of the FWA promoter of the same representative T1 plants displayed in b. Error bars represent the mean ± s.d. of 3 technical replicates. E represents early flowering and L represents late flowering. d Flowering time of T2 plants in wild-type, nrpe1-11, and the progenitor lines of the four late flowering T1 hex plants as indicated by the leaf number. The green dots in line T2-1 represent three late flowering T2 plants propagated to their T3 generation. e qRT-PCR of FWA expression level of one representative early and three late flowering plants in T2 progenitor lines in hex background, relative to that in nrpe1 background. Leaf number of the corresponding plant is indicated. E early flowering, M intermediate flowering, L late flowering. Error bars represent the mean ± s.d. of 3 technical replicates. f McrBC analysis of the FWA promoter of the same T2 progenitor lines displayed in e. Error bars represent the mean ± s.d. of 3 technical replicates. E represents early flowering and M indicates intermediate flowering. E early flowering, M intermediate flowering, L late flowering. g Flowering time of T3 plants in wild-type, nrpe1-11, and the progenitor lines of the three late flowering T2-1 morc hex line as indicated by the leaf number. E early flowering, M intermediate flowering, L late flowering. h qRT-PCR of FWA expression level of one early flowering and three representative late flowering plants in T3 progenitors derived from T2-1. Leaf number of the corresponding plant is indicated. Error bars represent the mean ± s.d. of 3 technical replicates. E early flowering, M intermediate flowering, L late flowering. i McrBC analysis of the FWA promoter of the same representative T3 plants displayed in i. Error bars represent the mean ± s.d. of 3 technical replicates. E early flowering, M intermediate flowering, L late flowering. Source data underlying Fig. 6 are provided as a Source Data file.
