Fig. 6: Immunofluorescence imaging of SLC30A10 protein constructs expressed in cultured HeLa cells.

WT = wild type (a–d); T95I = Thr95Ile (e–h); Δ105–107 (i–l) and L89P (m–p), HMNDYT1-causing variants reported previously56; empty = transfected with empty vector (q–t); NTC = non transfected control (u–x). Calnexin staining (in red) indicates the endoplasmic reticulum (ER). Color balance has been adjusted globally to improve the contrast between channels in overlay images; original unadjusted micrographs are provided as a Source data file.