Fig. 2: The preparation and characterization of Oxa(IV)@ZnPc and Oxa(IV)@ZnPc@M.

a Average diameters of Oxa(IV)@DOPA and Oxa(IV)@ZnPc measured by DLS. b TEM image of Oxa(IV)@ZnPc, this experiment was performed three times with similar results. c Flow cytometric analysis of the purity of BMMs cultured in L929-CM doubly stained with F4/80-FITC and CD11b-PE. Cellular uptake by BMMs (d) quantitatively determined by fluorescence spectra (n = 3 technical replicates, the experiment is representative of three independent experiments) and e observed by CLSM at 0.5, 1, 2, and 4 h incubation with Oxa(IV)@ZnPc and Cal@ZnPc nanoparticles, respectively, n = 3 technical replicates, the experiment is representative of two independent experiments. Scale bars = 30 µm. f Intracellular Oxa contents (g) ZnPc contents at 2 h incubation with different concentrations of Oxa(IV)@ZnPc (n = 3 technical replicates, the experiment is representative of three independent experiments). Cell viability at 2 h, 6 h, 12 h, and 24 h via CCK8 assay after incubation with h Oxa(IV)@ZnPc and i free Oxa at different platinum concentrations for 2 h (n = 3 technical replicates, the experiment is representative of three independent experiments). j Cellular uptake by BMMs observed by TEM at 2 h incubation with Oxa(IV)@ZnPc, this experiment was performed one time. k Live/Dead cell staining results of BMMs and Oxa(IV)@ZnPc@M at 2 h after drug loading, this experiment was performed two times with similar results. Scale bars = 50 µm. All data were presented as mean ± SD. Source data are provided as a Source data file.