Fig. 8: Long-term immune memory effects in 4T1-bearing mice. | Nature Communications

Fig. 8: Long-term immune memory effects in 4T1-bearing mice.

From: Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer

Fig. 8

a Schematic illustration of experimental. b Tumor growth curves of rechallenged tumors monitored by BLI after various treatments (1: PBS (+); 2: free Oxa; 3: Oxa(IV)@ZnPc; 4: Oxa(IV)@ZnPc@M; 5: BMMs + anti-PD-L1; 6: Oxa(IV)@ZnPc (+); 7: Oxa(IV)@ZnPc@M (+); 8: Oxa(IV)@ZnPc@M (+) + anti-PD-L1, “(+)” represent with laser irradiation, applied for all the studies in vivo) (n = 4 individual animals). c Representative in vivo bioluminescence images tracking the growth of intra-tibia injected Luc-4T1 cells (n = 4 individual animals). d Quantitative luminescence intensity of Luc-4T1 bone metastatic tumors at the last BLI (*p = 0.0245, *p = 0.0356) (n = 4 individual animals). e Tumor growth curves of rechallenged tumors monitored by caliper measurement (n = 8 individual animals, the volume of mice without tumors is recorded as zero, ***p = 0.0003, ***p < 0.0001). f Relative body weight changes after various treatment (n = 8 individual animals). g Overall survival curves of mice after various treatments. All values were presented as means ± SD. Statistical significance was calculated via ordinary one-way ANOVA with a Dunnett’s test. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source data file.

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