Fig. 4: Downregulation of MYST1 mediates the stimulatory effects of TGFβ on autophagy.
From: TGFβ promotes fibrosis by MYST1-dependent epigenetic regulation of autophagy
a, b Effects of siRNA-mediated knockdown of MYST1 on autophagy in healthy human dermal fibroblasts as assessed by quantification of the mRNA levels of ATG7 (n = 4 biological replicates per group and n = 3 for MYST1 siRNA + TGFβ), BECLIN1 (n = 4 biological replicates per group) and respective protein quantifications (n = 4 biological replicates per group). Confirmation of reduced mRNA (n = 4 biological replicates per group) (a) and protein (b; n = 4 biological replicates per group) levels of MYST1 by qRT-PCR and western blot. c EGFP/RFP-LC3 reporter activity upon knockdown of MYST1; representative images and quantification (n = 5 biological replicates per group). Horizontal scale bar, 5 µm. d, e mRNA expression of ATG7, BECLIN1, and MYST1 (n = 6 biological replicates per group) (d) and respective protein quantification (e) (n = 4 biological replicates per group) levels after adenoviral overexpression of MYST1 in healthy human dermal fibroblasts (e). All data are presented as mean ± IQR. p-values were determined by ANOVA one-way with Tukey’s multiple comparison post hoc test. See source data for more detailed information. rel.: relative, Ad: adenovirus. The results in panel c are part of a large set of experiments and share controls with Supplementary Fig. 8a. Data are presented separately just for the purpose of this figure.
