Fig. 6: Antibodies generated by intrarenal B cells were not selected for allo-HLA reactivity.
From: Innate-like self-reactive B cells infiltrate human renal allografts during transplant rejection
a A violin plot showing distribution of mutations in the variable region of immunoglobulin heavy chains, grouped by tissue source and the Ig class-switch state. b t-SNE plots as in Fig. 6, with data points labeled with their clonal family. “-“ means antibody genes were not sequenced; “0” means the cells did not share their clonotype with others; and 1-22 indicate that the cells shared a clonotype with other B cells with the same number. c A violin plot showing distribution of mutations in recombinantly expressed antibodies, grouped by tissue source and Ig class-switch state. d A heatmap showing the HLA-binding assay result. Each column represents each antibody, and maximum NBG ratio within class-1 or class-2 beads are shown. The header label indicates patients from whom the antibodies were derived, serum DSA positivity, and whether the observed reactivity was above the positivity threshold. e A plot showing the change in HLA binding when the antibodies were tested in negative control human serum. All the positive intrarenal antibodies in (d) were tested, and data points of the same antibodies were connected by lines.
