Fig. 2: Comparison of striatal and cortical datasets reveals a common astrocyte maturation signature.
From: Extensive transcriptional and chromatin changes underlie astrocyte maturation in vivo and in culture
a Approach used for MACS (magnetic bead assisted cell sorting) enrichment and bulk RNA-Sequencing (RNA-Seq) analysis of immature astrocytes from postnatal day 4(P4) cortex and of mature astrocytes from adult cortex (age 2 months (2m), range 6–10 weeks (6–10w)). b, c Volcano plot (b) and Venn diagrams (c) show genes differentially expressed between cortical astrocyte preparations from P4 and adult brain. Maturation-regulated genes identified by scRNA-Seq of striatal astrocytes (see Fig. 1f) are highlighted in (b). Venn diagrams (c) show overlap of genes significantly regulated between early postnatal and adult astrocyte preparations in both striatal scRNA-Seq and cortical bulk RNA-Seq datasets. Biological and technical differences between the analyses may contribute to the differences in genes detected in the two datasets, as indicated in (c). Differential genes: DESeq2 analysis, each n = 3; two-sided Wald test with Benjamini–Hochberg correction; significance threshold: adjusted p-value (padj) ≤ 0.05, absolute log2(fold change)(log2FC) ≥ 1.See also Supplementary Data 2.
