Fig. 2: MAFF induction under hypoxia is regulated by HIF-1.
From: The HIF target MAFF promotes tumor invasion and metastasis through IL11 and STAT3 signaling
a MAFF mRNA expression was elevated under hypoxia in breast cancer cell lines. n = 3 biological replicates. Unpaired t-test. b While basal expression of MAFF was different, hypoxia increased MAFF expression in all breast cancer cell lines. c, d Knocking down HIF1A or ARNT (HIFβ) prevented MAFF mRNA and protein induction under hypoxia. One-Way ANOVA with multiple comparisons. e ChIP assay was performed to identify a HIF-1 binding site on the MAFF promoter (HRE8 and 9) (TSS: transcription start site). n = 4 biological replicates. Unpaired t-test. f Luciferase reporter assays showed that transcription activity of HRE8 or HRE9 was increased under hypoxia compared to mutated HRE8 or HRE9. pGL4-HRE, which contains 4x HRE regions, was used as a positive control. n = 3 biological replicates. Unpaired t-test. Graphs represent the mean per group and error bars represent the SEM. ns: not significant.
