Fig. 6: MAFF-mediated IL11 regulates tumor cell invasion.
From: The HIF target MAFF promotes tumor invasion and metastasis through IL11 and STAT3 signaling
a Knocking down IL11 significantly decreased invasive phenotypes of MDA-MB-231 through collagen (upper panels) and Matrigel-coated transwell membranes (lower panels). n = 3 biological replicates, Unpaired t-test. b The rescue effect of IL11 expression on decreased tumor cell invasion was evaluated in MDA-MB-231 cells with or without MAFF knockdown. n = 3 (collagen assay, upper panels) or 4 (transwell membrane assay, lower panels) biological replicates. One-Way ANOVA with multiple comparisons. c STAT3 activation was evaluated in MDA-MB-231 cells treated under normoxia or hypoxia by Western blotting. Hypoxia increased phospho-STAT3 (Tyr705), while MAFF knockdown inhibited its induction both under normoxia and hypoxia. d, e The downstream effect of IL11 on STAT3 activation was determined after IL11 was knocked down (d) or overexpressed with or without MAFF inhibition (e). Phospho-STAT3 expression was investigated by Western blotting. f, g Endothelial tube formation assay was performed using GFP-labeled HUVEC cells. Cell culture media were transferred from MDA-MB-231 cells infected with IL11 Cas9-CRISPR (f) or MAFF shRNA with or without IL11 overexpression (g). n = 3 biological replicates, Unpaired t-test (f) or One-Way ANOVA with multiple comparisons (g). Graphs represent the mean per group and error bars represent the SEM.
