Fig. 3: NAC downregulates DAF-16 and SKN-1-dependent transcription.

a The most enriched categories (gene ontology) among genes downregulated by NAC. Numbers indicate the number of genes per a category; see Supplementary Data 1 for the full list of differentially regulated genes, and Supplementary Fig. 3 for statistics. b Regulation of representative DAF-16 dependent genes by LB, DB or DB + NAC. Graphs show RPKM of three independent experiments from RNA-seq. Box plots indicate median (middle line), 25th, 75th percentile (box) and 5th and 95th percentile (whiskers) as well as maximum, minimum and mean (single points).See Supplementary Data 1 for statistics. c Statistically significant overlap between the sets of genes downregulated by NAC and 500 top-ranked DAF-16- (189 genes, RF = 5.6, p value = 4.28e−93) and SKN-1- (92 genes, RF = 5.9, p value = 3.01e−47) dependent genes under normal growth conditions. RF – representation factor is the number of overlapping genes divided by the expected number of overlapping genes drawn from two independent groups and p is normal approximation of hypergeometric probability. d NAC (5 mM) shortens the lifespan of daf-16 worms. Average percentage change ±SD of the lifespan relative to untreated control is indicated in red (n = 304(untreated), 305(5 mM NAC)).