Fig. 5: Neutralization of GII.4 and GII.17 in human intestinal enteroid system.
From: Broadly cross-reactive human antibodies that inhibit genogroup I and II noroviruses
HuNoV was mixed with an equal volume of medium or dilutions of the indicated antibody, and then incubated at 37 °C for 1 h. Human intestinal enteroid monolayers were inoculated with each virus-antibody mixture for 1 h at 37 °C in the presence of 500 μM GCDCA. The monolayers were washed twice and then cultured in the presence of GCDCA for 24 h. Compiled data from two experiments are presented. Each data bar represents the mean ± SD of six wells of inoculated HIE monolayers. Error bars denote standard deviation and individual points are shown (n = 12 for IgA and Fab and n = 6 for GII.4_control). Percent reduction in viral genome equivalents (GEs) relative to medium (100%) was determined. The dotted line represents 50% neutralization. Significance relative to the control was determined using two-tailed Student’s t test. Exact ρ values from left to right: (A) ***ρ = 1.10E−16, 2.30E−13, 4.78E−08, 9.70E−07, *ρ = 0.03; (B) ***ρ = 1.3E−13, 1.13E−11; (C) all differences are n.s., not significant; (D) ***ρ = 2.45E−09, 4.13E−07.
