Fig. 9: Dynamic light scattering of mAb NORO-320 and GII.4 Sydney VLP.
From: Broadly cross-reactive human antibodies that inhibit genogroup I and II noroviruses
The hydrodynamic diameters of treated or untreated GII.4 HuNoV VLPs were measured using dynamic light scattering (DLS) on a ZetaSizer Nano instrument (Malvern Instruments, UK). A Complete dynamic light scattering profile of the four tested conditions: GII.4 HOV VLP alone or in complex with 22D2 IgG, NORO-320 IgA, or Fab at a molar ratio 1:1 or 1:10. A dengue virus-specific antibody, 2D22 IgG, was used as a control at a molar ratio 1:10. B Average diameters were calculated for each sample condition using Zetasizer software. Samples were diluted to a final concentration of 330 nM for each component in phosphate-buffered saline pH 6, and a 3,300 nM concentration of NORO-320 Fab was prepared for the condition labeled VLP:NORO-320 Fab (1:10). Individual data points are presented as black or gray symbols. Three × 12 measurement runs were performed with standard settings (refractive index 1.335, viscosity 0.9, temperature 25 °C). Each data bar represents the mean ± SD of n = 3 independent experiments.
