Fig. 5: Spatial characterizations of sterol lipids in the mouse brain.

a Dissection of functional regions of mouse brains (6-week; n = 6 biologically independent samples for each group). Each sample was analyzed once by LC-IM-MS. b Numbers of sterol lipids identified in the brain regions. c The distributions of shared and unique sterol lipids in the brain regions. Each column represents the number of sterol lipids identified in the specific brain regions, which were shown as black solid dots in the lower part of the plot. d Absolute concentrations of sterol lipids in 14 sterol subclasses measured in the brain regions. The sterol concentrations were normalized to tissue wet weight (mg). e Hierarchical clustering analysis of sterol lipids in mouse brain regions (n = 149). f–h Percentages of sterol subclasses in clusters 1–3 and concentration distributions of representative sterol subclasses in mouse brain regions. Data are presented as mean values +/− SD (n = 6 biologically independent samples for each group).