Fig. 2: Auditory input enhances tactile-evoked somatic action potentials in layer 2/3 pyramidal neurons in vivo.
From: Auditory input enhances somatosensory encoding and tactile goal-directed behavior
a Left, Schematic of experimental design. Mice were previously habituated to head fixation before whole-cell patch-clamp recordings were performed from L2/3 pyramidal neurons in forepaw S1 in the awake state. Right, L2/3 pyramidal neuron filled with fluorescence biocytin for reconstruction and example voltage response to injected current steps (50 pA). (Left) Overlay of individual trials and raster of action potentials from a typical neuron and (right) average evoked action potentials per trial in all recorded neurons in response to (b) tactile stimulus (Wilcoxon matched-pairs signed rank test; p = 0.027; pshuffled = 0.782; 27 trialsav), (c) paired auditory and tactile stimulus (two-tailed Wilcoxon matched-pairs signed rank test; p = 0.008; pshuffled = 0.927; 28 trialsav) and (d) auditory stimulus (two-tailed Wilcoxon matched-pairs signed rank test, p = 0.75; pshuffled = 0.41; 21 trialsav). n = 17 neurons, 8 mice. Base = baseline. Colored trace, single example voltage traces for tactile (black), paired auditory and tactile (red), auditory (blue) stimulus. e Average evoked action potentials during paired auditory and tactile stimulus (red) normalized to tactile stimulus (black) (p = 0.013). Wilcoxon matched-pairs signed rank test. n = 17 neurons, 8 mice. f Mean somatic voltage response during tactile (black), paired auditory and tactile (red), and auditory (blue) stimulus (n = 17 neurons, 8 mice; One-way ANOVA Friedman test, p < 0.0001; Dunn’s multiple comparisons test. p = 0.0002, p > 0.999, p < 0.0001). Error bars represent S.E.M. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
