Fig. 4: NEK2 positively correlates and interacts with PD-L1 in pancreatic cancer.
From: NEK2 inhibition triggers anti-pancreatic cancer immunity by targeting PD-L1
a Western blot analysis of NEK2 and PD-L1 in clinical pancreatic tissue samples from patients (n = 20) (N: Normal pancreatic tissue; T: Pancreatic tumor tissue). b–d Representative images and statistical results of IHC staining of NEK2 and PD-L1 in a tissue microarray (n = 156). e–g Cell lysates from SW1990, KPC, and CFPAC-1 separately analyzed by IP and Western blotting using the antibodies indicated. Representative image is shown n = 3 independent experiments. h GST-pull down assay of NEK2-His and GST-PD-L1 protein. Representative image is shown n = 3 independent experiments. i Representative images of individual immunofluorescence staining of NEK2 and PD-L1 interaction in KPC cells by Duolink assay. The red dots (NEK2/PD-L1 interaction) indicate their interaction. Representative image is shown n = 3 independent experiments. The Spearman correlations and p-values by Spearman’s test are indicated in d.
