Fig. 7: Isogenic iPSC lines reduce among- and within-line variability of organoid cultures.
Analysis of among-line (a)–(e) and within-line (f)–(j) variance for individual sphere potency (a), (f) and area (b), (g), as well as GFAP+ cells (c), (h), IBA1+ cells (d), (i), and SAA + area (e), (j) within mature organoid cultures from two to three independent biological differentiation replicates for each line. a–e left, Mean and SEM of fold change from batch average, along with points for individual spheres (a), (b) or cultures (c), (e). Right, Mean and SEM among non-isogenic and isogenic lines with points for each unique iPSC line. Significance assessed by F-test for Equality of Variances, with group variances (σ2) indicated at bottom, for n = 5 non-isogenic, 6 isogenic lines: (a) F = 2.0 × 105, P = 6.8 × 10−13; (b) F = 2.0 × 105, P = 6.8 × 10−13; (c) F = 4.37, P = 0.137; (d) F = 106.33, P = 1.0 × 10−4; (e) F = 18.90, P = 0.006. f–j, left, Mean and SEM of fold change from batch average within-line, along with points for individual spheres (f), (g) or cultures (h)–(j). Right, Mean and SEM of within-line variances for non-isogenic and isogenic lines with points for each unique iPSC line. Significance assessed by one-way ANOVA for n = 5 non-isogenic, 6 isogenic lines: (f) (non-isogenic vs isogenic) 79.9 ± 24.0 vs 7.0 ± 2.1, F = 11.21, P = 0.009; (g) 0.052 ± 0.017 vs 0.007 ± 0.002, F = 8.825, P = 0.016; (h) 0.603 ± 0.198 vs 0.030 ± 0.004, F = 10.25, P = 0.011; (i) 0.711 ± 0.401 vs 0.114 ± 0.035, F = 2.70, P = 0.135; (j) 0.328 ± 0.095 vs 0.015 ± 0.003, F = 13.2, P = 0.005. Colors indicate individual iPSC lines.
