Fig. 6: WNK1 regulates intracellular Cl− in macrophages.
From: Chloride sensing by WNK1 regulates NLRP3 inflammasome activation and pyroptosis
Quantification of intracellular Cl− (a–c), K+ (d–f), IL-1β release (g–i) and propidium iodide uptake (j–l) in LPS-primed (4 h) primary Wnk1+/+ LysMCre+ (WNK1 WT), Wnk1flox/flox LysMCre+ (WNK1 KO), or WNK463-treated wild type BMDMs following treatment with nigericin (a, d, g, j), ATP (b, e, h, k), or Imiquimod (c, f, i, l) in normal medium. P values of a 0.001839, 0.00135; b 0.01758; c 0.001793; d 0.001072; e 0.00327; f 0.00135; g 0.4217, 0.00072976, 0.0323, 0.00101, 0.00219, and 0.00345; h 0.058, 0.0594, 0.000284, 0.000056, 0.0000013, and 0.0000127; i 0.0556, 0.0146, 0.01339, 0.0000265, 0.000953, and 0.000084; j 0.00000030; k 0.00242; l 0.000111. Results are representative of at least three independent experiments performed in duplicate or triplicate. Error bars in a–l are presented as mean values ± standard deviation (S.D.), with n = 3. Two-sided Student’s t test, *p < 0.05, **p < 0.005, ***p < 0.0005.
