Fig. 3: Bisected X segments are stained with markers associated with transcription during male meiosis. | Nature Communications

Fig. 3: Bisected X segments are stained with markers associated with transcription during male meiosis.

From: Bisection of the X chromosome disrupts the initiation of chromosome silencing during meiosis in Caenorhabditis elegans

Fig. 3

a Mid-pachytene male nuclei stained with DAPI (blue), X-right FISH (red), and antibody against H3K9me2 (green). b Averages ± SEM of the relative H3K9me2 signals in wild-type and YBT7 nuclei on HIM-8-marked body vs. an autosome. n = 17 and 36 nuclei for WT and YBT7 respectively. p value = 0.00003 by the two-tailed Mann–Whitney test. c Mid-pachytene male nuclei stained with DAPI (blue), X-right FISH (red), and antibody against RNAPII (green). d Averages ± SEM of the relative RNAPII signals in wild-type and YBT7 nuclei on HIM-8-marked body vs. an autosome. p value = 0.000004, by the two-tailed Mann–Whitney test. N = 62 and 34 nuclei for WT and YBT7 respectively. ****p < 0.0001, by the two-tailed Mann–Whitney test. Scale bar = 3 μM.

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