Fig. 6: Inhibition of CK2 extends the worm lifespan.

a Honeycomb diagram displays iGPS-predicted kinases whose target motifs were significantly (one-sided hypergeometric test, p < 0.05) enriched among the hypo-phosphorylated sites in the daf-2 mutant. Kinase groups are depicted in different colors. Bold black highlights the kinases reported to regulate lifespan. See statistics in Source data. b CK2 consensus motifs overrepresented in the hypo-phosphorylated peptides in the daf-2 mutant. An online service pLogo⁵² (https://plogo.uconn.edu/) was used to visualize the statistical significance of the frequently occurred motifs among the hypo-phosphorylated sequences in daf-2. The phosphosites were fixed at position 0. All quantified phosphopeptides were taken as the background sequences. The red horizontal lines correspond to p = 0.05. c CK2 knockdown in adulthood significantly extended the lifespan of WT worms. kin-3 or kin-10 encodes the catalytic or regulatory subunit of CK2, respectively. Lifespan assays were performed at 25 °C, with 50 ng/μl FUdR supplied in plates. d RNAi of kin-3 or kin-10 from adult day 1 for 24 h significantly extended the lifespan of WT worms. Lifespan assays were performed at 20 °C without FUdR. e Feeding WT with TBB, the highly selective inhibitor of CK2, from adult day 1 for 24 h significantly extended the worm lifespan. Lifespan assays were performed at 20 °C, with 50 ng/μl FUdR supplied in plates. f TBB treatment from adult day 1 for 24 h significantly extended the lifespan of C. elegans. Lifespan assays were performed at 20 °C without FUdR. *p < 0.05, ***p < 0.001, two-sided log-rank test, n > 70 worms per strain. See survival statistics in Supplementary Dataset 4.