Fig. 7: Phosphoproteomics analysis supports concerted regulation of longevity from multiple pathways upon reduced IIS.
From: Insulin signaling regulates longevity through protein phosphorylation in Caenorhabditis elegans
daf-2(lf) induced extensive phosphorylation changes on components of the IIS pathway, translational machinery, reproductive system, and kinome. Phosphorylation changes at AKT-1 pT492, EIF-2α pS49, and CDK-1 pT179 all affect lifespan, while their parent proteins and the encoding mRNAs show no or little change in the daf-2 mutant, except for AKT-1. The proteins involved in reproduction and lifespan determination are likely regulated through phosphorylation by IIS, since no clear pattern of changes is evident based on analysis of their mRNA or protein levels. Similarly, reduction in IIS may lower the activity of pro-ageing kinases, and the regulation pattern cannot be inferred from mRNA41 or protein6 abundance changes. ↑ increase or extend, ↓ decrease or shorten, — no significant change.
