Fig. 1: Slitrk5 is expressed in osteoblasts and negatively regulates osteoblastogenesis.
From: SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts
a Expression of Slitrk5 mRNA in indicated cells or tissues. Data are presented as mean ± s.d. n = 4 biologically independent samples. b Immunofluorescence staining of mouse femur sections with anti-beta-galactosidase and OPN antibodies, demonstrating the expression of Slitrk5 in osteoblasts. Data are representative of two independent experiments, scale bar = 200/10/10 µm. c, d Primary osteoblasts from WT and Slitrk5−/− mice were cultured in osteoblast differentiation medium. Mineralization activity was assessed by alizarin red staining (c) at day 14 of differentiation. Alkaline phosphatase (ALP) activity was measured at day 8 of differentiation (d). Data in (d) are presented as mean ± s.d. n = 5 biologically independent samples, two-tailed unpaired t test. e RT-PCR analysis of Slitrk5 and osteoblast marker genes expression at day 6 and day 12 of differentiation in WT and Slitrk5−/− osteoblasts during differentiation. n = 4 biologically independent samples, two-tailed unpaired t test. Data are presented as mean ± s.d.
