Fig. 3: SLITRK5 interacts with SHH and PTCH1.
From: SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts
a Primary osteoblasts from WT and Slitrk5−/− mice were treated with the indicated doses of Purmorphamine and cultured in osteoblast differentiation medium. ALP activity was measured at day 3 of differentiation. Data are presented as mean ± s.d. n = 6 biologically independent samples. b Co-immunoprecipitation of Flag-SLITRK5 and SHH in HEK293T cells. Data are representative of four independent experiments. c Co-immunoprecipitation of SHH and Flag-tagged SLITRK5 truncation mutants in HEK293T cells. Data are representative of three independent experiments. d ELISA assay showing the interaction of hSLITRK5-ECD and SHH. e Surface Plasmon Resonance analysis of the binding of SHH to SLITRK5. SHH was injected over a SLITRK5 surface at 125, 62.5, 31.25, 15.625, and 7.8125 nM. f, g Co-immunoprecipitation of Flag-SLITRK5 and HA-PTCH1 in HEK293T cells with BSA or SHH treatment. Data are representative of two independent experiments. h Co-immunoprecipitation of PTCH1 and Flag-tagged SLITRK5 truncation mutants in HEK293T cells. Data are representative of three independent experiments.
