Fig. 4: SLITRK5 regulates SMO ciliary enrichment upon SHH stimulation.
From: SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts
a Immunostaining for SLITRK5 (anti-Flag antibody, Green) and acetylated tubulin (Red) in osteoblasts treated with BSA or SHH for 4 h. Data are representative of three independent experiments, scale bar = 5 µm. b Quantification of the relative fluorescence intensity of ciliary SLITRK5-flag. n = 40–50 cells per group. Data are presented as mean ± s.d, two-tailed unpaired t test. c, d Ciliary localization of SMO-GFP (c) and PTCH1- Flag (d) in WT and Slitrk5−/− osteoblasts treated with BSA or SHH for 4 h. Data are representative of three independent experiments, Scale bar = 5 µm. e, f Quantification of the relative fluorescence intensity of ciliary SMO-GFP (e) and PTCH1-Flag (f) in WT and Slitrk5−/− osteoblasts treated with BSA or SHH for 4 h. n = 100–300 cells per group. Data are presented as mean ± s.d, One-way ANOVA (P < 0.0001) followed by a Tukey’s post hoc test.
