Fig. 4: Analysis for hei10 heterozygote.

a 3D-SIM image and example SC measurements from a late pachytene cell of the hei10-2 +/− line. Representative image shown. Data were collected from 164 late pachytene bivalents for further analysis. Scale bars = 5 µm. b–g Experimental data (left) and simulation outputs (right) for hei10 +/−, with simulated HEI10 levels 0.6-times WT (data from 164 bivalents from 40 cells from 3 plants). Crossovers (COs) in the experimental data are defined as the positions of late-HEI10 foci. b Number of COs per bivalent. c–e CO positions along bivalents. (Data from 266 COs). c All COs. d Bivalents with single CO. e Stacked histograms for double COs, showing positions of 1st/2nd CO. f Histograms of intensities of CO HEI10 foci, relative to sum of all foci within a cell, colour-grouped according to CO number per bivalent (inset). g Distribution of spacing between adjacent COs. Source data are provided as a source data file.