Fig. 2: Location of human cholinergic progenitor (NTRK2+ CLDN11+) and chromaffin (TH+) cells within the postnatal human adrenal gland (AG). | Nature Communications

Fig. 2: Location of human cholinergic progenitor (NTRK2+ CLDN11+) and chromaffin (TH+) cells within the postnatal human adrenal gland (AG).

From: Single-nuclei transcriptomes from human adrenal gland reveal distinct cellular identities of low and high-risk neuroblastoma tumors

Fig. 2

a tSNEs representing the expression of indicated genes in human cholinergic progenitor (pink) and chromaffin (blue) populations. The bars next to the tSNEs illustrate the expression measured as the logarithm of the read counts per 10,000. b–e Overview of tile-scanned images (×20) of postnatal human adrenal glands (AG) at indicated age. Scalebar of overview: 200 μm, zoom of boxed image: 10 μm. b–d RNAscope in situ hybridization (ISH) for TH (green), CLDN11 (red), and NTRK2 (white) mRNA and counter stained with DAPI (blue). NTRK2+ CLDN11+ double positive cells were found in adrenal capsule and medulla exclusive from TH positive cells. e RNAscope ISH of a 4-year-old AG labeled with for TH (green), CHRNA7 (red), and CLDN11 (white) mRNA and nuclear counter-stain (DAPI) as indicated. For all RNAscope experiments, the signal distribution patterns and cell morphological features were shown by the different combination of probes and independently reproduced three times on different samples.

Back to article page