Fig. 4: 108600 induces G2/M arrest and apoptosis of TNBC cells and organoids.

A MDA-MB-231 cells were treated with the indicated concentrations of 108600, stained with propidium iodide (PI) and analyzed by flow cytometry. Statistical differences were calculated using Dunnett’s test (two-sided). A p value of less than 0.05 is indicated by an asterisk and is regarded as statistically significant. B CTG1883 TNBC organoids were treated with 108600 (0.5 µM), stained with PI and analyzed by flow cytometry. All data represent mean values ± standard deviation obtained from three independent biological replicates. Statistical differences were tested using t-test (two-sided). A p value of less than 0.05 is indicated by an asterisk and is regarded as statistically significant. C MDA-MB-231 cells were treated with 108600 for 24 or 48 h. Lysates derived from these cells were subjected to western blot analysis and probed for the expression of total or cleaved PARP (top), which is inhibited by a 2 h pretreatment with the ZVAD (50 µM) caspase inhibitor (bottom). Samples derive from the same experiment and the blots processed in parallel. The blots are representative of three independent experiments. D CTG1883 organoids were treated with 108600 for 48 h. Lysates from these cells were probed for the expression of total or cleaved PARP and caspase 3. All westerns were performed on the same gel. The blot is representative of three independent experiments. Source data are provided as a source data file.