Fig. 7: 108600 and paclitaxel synergize to inhibit established TNBC metastases.

MDA-MB-231/LM2-4 mCherry cells were used to generate tumors in the mammary fat pads of 7-week-old NSG mice. Surgery to remove the primary tumor was performed and all mice were imaged by IVIS for the presence of metastases. A Schema of the experiment. Mice with metastases were randomized into four treatment groups (n = 10–12 per group) and metastatic disease was monitored by serial IVIS imaging. B Serial IVIS imaging of mice in the four treatment groups. C Total luminescence in the region-of-interest (lung) window for the four groups at time of treatment randomization (1st IVIS), showing no statistically significant difference in lung metastases burden (Dunnett’s test, two-sided). D The ratio of luminescence intensity compared to baseline luminescence (C) for each treatment group at serial timepoints indicated in the schema. Ratios were calculated at each IVIS imaging. P values were calculated with Dunnett’s (two-sided) and Tukey’s tests (two-sided) in comparison to the value for the DMSO-treated group. Each data point represents data obtained for an individual animal, with the top and bottom of the box corresponding to the 75th and 25th percentiles, respectively. Data points that are higher or lower than 1.5 times that of the interquartile range within the box were regarded as outliers.