Fig. 6: Depletion of U2AF65 rather increases splicing of pre-mRNAs with SPF45-dependent short introns.
From: SPF45/RBM17-dependent, but not U2AF-dependent, splicing in a distinct subset of human short introns
a The co-depletion of U2AF65 and U2AF35 proteins from HeLa cells was observed by a Western blotting. Displayed blots are representative of three independent experiments. b After the siRNA transfection, HeLa cells were cultured for short time (4 h) to effectively observe splicing stimulation, and splicing efficiencies of the indicated four mini genes were analyzed by RT-PCR. Means ± SEM are given for three independent experiments and two-tailed Student t-test values were calculated (AdML intron: p = 0.0067 for Control vs U2AF65 siRNA; HNRNPH1 intron: p = 0.0038 for Control vs U2AF65 siRNA; EML3 intron: p = 0.0116 for Control vs U2AF65 siRNA; MUS81 intron: p = 0.0273 for Control vs U2AF65 siRNA. *P < 0.05, **P < 0.01. Source data of all the above panels are provided as a Source Data file.
