Fig. 1: Serial cryoFIB/SEM volume imaging of the entire SARS-CoV-2-infected cell.
From: Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress
A–D Representative cryoFIB/SEM slices of a SARS-CoV-2-infected cell at the cell periphery (A), cytoplasm (B), cell nucleus (C), and invagination of cytoplasm into the nuclear space (note, from a different cell) (D). Scale bars, 500 nm in A–C, 1 µm in D. Black and red arrows, extracellular and intracellular virus particles, respectively; blue arrows, nuclear pores; pink arrows, complex membrane compartment; dashed purple arrow, invagination path. V vesicles, T tunnels, Nuc nucleus, Cyto cytoplasm, ER endoplasmic reticulum. E Surface rendering of the segmented volume of SARS-CoV-2-infected cell shown in A–C. Segmented organelles and virus particles are labeled with the colors indicated. The dashed lines (E, top left panel) indicate the positions of slices shown in A–C, respectively.
