Fig. 3: SARS-CoV-2 cytoplasmic viral assembly.
From: Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress
A, B CryoFIB/SEM images of two sequential slices separated by 80 nm. Black arrows point to virus particles in a vesicle (V). Purple arrow points to small electron-dense material lining the outside of virus-containing vesicle. C Tomographic slice of cryoFIB lamella depicting SARS-CoV-2 assembly, with DMV portals (yellow arrow), assembling viruses (blue arrow), assembled virus (black arrow), viral spikes on SMV membranes (red arrows), transporting vesicles around the assembly site (pink arrow), and a nucleopore (black arrowhead). D Density segmentation of C, displaying three virus particles (black arrows) and two assembly sites (blue arrows). E An enlarged view (at a different angle) of boxed area in C, showing assembled virus (black arrow), assembling viruses (blue arrows), spikes (red arrows), and spike-containing vesicles (pink arrows). F Large intracellular virus-containing vesicle (LVCV) full of readily assembled viruses. G Subtomogram average of viral spikes of intracellular viruses from cell lamellae at 16 Å resolution, fitted with an atomic model of spike trimer (PDB 6VXX)16. Scale bar is 300 nm in A–C and 100 nm in E, F.
