Fig. 1: p300 phase separates to condensates in living cells. | Nature Communications

Fig. 1: p300 phase separates to condensates in living cells.

From: Nuclear condensates of p300 formed though the structured catalytic core can act as a storage pool of p300 with reduced HAT activity

Fig. 1

a Schematic of p300FL and p300BRPHZ. b Representative images of HeLa cells expressing YFP-p300FL. Scale bar, 5 µm. c FRAP curve of YFP-p300FL was obtained from averaging data from six HeLa cells. Error bars represent SEM. n = 6 cells examined over three independent experiments. d Representative FRAP images of YFP-p300FL expressed in HeLa cells. The images were taken before and after photobleaching at indicated time points. The bleached condensate is indicated by a white arrowhead. Scale bar, 5 µm. e FRAP curve of YFP-p300BRPHZT condensates in HeLa cells. The FRAP curve was obtained from averaging data from four cells. Error bars represent SEM. f A representative image of a sample (from at least three replicates) containing 13 µM p300BRPHZ and 12% PEG3350 (PEG) in a 100 µm × 100 µm square region under a microscope.

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