Fig. 7: A pool of p300 co-localizes with H3K27me3. | Nature Communications

Fig. 7: A pool of p300 co-localizes with H3K27me3.

From: Nuclear condensates of p300 formed though the structured catalytic core can act as a storage pool of p300 with reduced HAT activity

Fig. 7

a–d H3K27ac and H3K9ac western blot analysis of the reaction mixtures containing p300BRPHZ and an equimolar amount of NCP as a substrate. Reactions were quenched by rapid freezing and the addition of SDS-loading buffer at indicated time points. The intensity of bands is quantified, normalized to the +SIRT2/+PEG band at 2 min, and shown in (b) and (d). e Heatmaps of H3K4me3, H3K27me3, H3K27ac, H3K18ac, and Flag-p300BRPHZT, ChIP-seq signals centered on p300-H3K4me3 and p300-H3K27me3 binding sites in a ±20 kb window in H1299 cells stably expressing FLAG-p300BRPHZT. Heatmaps are ranked by H3K4me3 (upper panel) or H3K27me3 (lower panel). The color keys represent ChIP-seq densities normalized to total reads.

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