Fig. 5: Characteristics of Pc-G4 Pyr dendrimer complexes and their complement responses.

a A typical NTA 2D plot of relative light scattering intensity of Pc-G4 Pyr particles versus the estimate of the nanoparticle size. The experiment was repeated three times with identical distribution profile. A hydrodynamic size of 46 ± 3 nm (mean ± s.d.) was calculated from three independent determinations. b A representative transmission electron micrograph of Pc-G4 Pyr dendrimer complexes (left), and magnified view of the highlighted region (right). The experiment was repeated three times with identical results. c, d Lack of complement activation by Pc-G4 Pyr dendrimer complexes (3.5 mgmL⁻¹) in a typical human plasma determined through measurements of fluid-phase C5a and sC5b-9. A commercially available sulfated polystyrene nanoparticle suspension (PS) of 62 ± 6 nm (polydispersity index = 0.03 as determined by laser light scattering) was used as positive control for complement activation (final nanoparticle concentration = 3.5 mgmL⁻¹). In c, d, each bar represents the mean ± s.d. of three separate experiments, and each dot indicates the mean of three technical replicates. Indicated p values are derived from unpaired two-sided student t-test. Source data are available in Source data file.