Fig. 5: Further dissection of skyblue implicates cytotoxic lymphocyte subtypes in MIS-C.

A Defining NK cell and CD8⁺ T cell subtypes in skyblue (Supplementary Data 19). The x-axis represents the OR for the enrichment of skyblue for specific cytotoxic cell subtypes and the y-axis the signatures used. Cell one and cell two refer to the cell subtypes being compared to generate the DE signatures projected onto skyblue and the direction of the bar is shown towards the upregulated cell type in the comparison. The color and length of bars represent the OR of the enrichment test as defined by the x-axis and the legends. For NK cells, we used signatures from Yang et al., Nat. Commun., 2019 (labeled NK cells), and from Collins et al. Cell 2019 (labeled CD56^dim NK subtypes). For CD8⁺ T cells, we used signatures from Wherry, et al. Immunity, 2007 (labeled CD8⁺ T cells), and from Beltra et al. Immunity, 2020 (labeled CD8⁺ Tex subtypes). Detailed descriptions of the subtypes can be found in the Methods. B–E Skyblue key drivers measures. The x-axes of the panels represent the key driver genes. B Key driver analysis results (hypergeometric test).The y-axis of this panel is the −log₁₀(Bonferroni adjusted p-value) of key driver analysis (Supplementary Data 21). The red dashed line is the significance threshold at −log₁₀(0.05). C Related disease differential expression. In this panel, the y-axis is the disease name corresponding to Fig. 4. The color represents the direction in the comparison as defined in the legend (defined per the associated reference). D Cell-type-specific expression of key driver genes (Supplementary Data 22). The y-axis is the cell type signatures from the following references: 1 = Newman et al. Nature Methods, 2015; 2 = Park et al. Science, 2020; 3 = Wilk et al. Nature Medicine, 2020; 4 = Liao et al. Nature Medicine, 2020; and 6 = Szabo et al. Nature Communications, 2019. E CD8⁺ T cell and NK cell subtype-specific signatures. The y-axis is the cell-type name corresponding to A and the color represents the direction in the comparison as defined in the legend (defined per the associated reference).